Saturday, November 30, 2013

The Use Of Positive And Negative Controls To Determine The Macromolecules Present In An Unknown Solution

unravel # 6130, Wednesday, 7-10 PM, B2-151 Lab performed: September 21st, 2011 Introduction E nice living thing is dependent on large mixed molecules, cognize as macromolecules. The objective of this research laboratoryoratory was to right refer which macromolecules the unknown solution was comprised of using diverse substances as experimental view ass. There are four major types of biological macromolecules - carbohydrates, lipids, proteins, and nucleic acids - made up of elements such as carbon, hydrogen, oxygen, nitrogen, sulfur, and match in various combinations. Macromolecules are essential for survival; they show geomorphological support, a source of stored fuel, the ability to store and obtain heritable information, as well as the ability to further up biochemical reactions; hence their importance in biological systems is notable. (Prakash, 2008). The control samples have a known outcome; in this lab both incontrovert ible and disallow controls were used to determine the indistinguishability of the unknown solution. The macromolecules being well-tried in this lab were carbohydrates (monosaccharides and polysaccharides) and proteins.
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To identify the figurehead of these macromolecules in a substance, three different metrical unit races were performed. Lugols iodine solution was used for acknowledgment of starch and animal starch - polysaccharides - in the twelve solutions. A positive outcome of the turn up results in a colour reposition; no-account-black in the presence of starch and a red-brown in the presence of glycogen. A negative outcome results in no colour st! ir and all solutions remain a very pale jaundiced (Pavia, 2005). Benedicts solution was used to identify the presence of reducing sugars; the aldehyde operable group is the part that reacts in the test. A positive power of the test is the formation of a benighted precipitate of the blue solution, ranging from yellow-green to red-brown (Hequet and Abidi, 2006). Lastly, the Biuret solution was used to test for the presence of... If you ask to get a full essay, sound out it on our website: BestEssayCheap.com

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